Activities of the Biological Resource Centre

The Biological Resource Centre (CRB) is charged with storage of all the patient tissue samples which are considered by clinicians to be useful. All of these patients have signed consent forms and samples of their plasma, serum and whole blood are also stored. In addition the CRB keeps samples of public health importance and those collected in clinical research projects.      

Team members

• Lacroix Ludovic, Biologist/Team leader
• Jacques Nathalie, Laboratory manager
• Saulnier Patrick, Biologist engineer
• Jovelet Cécile, Postdoctoral researcher
• Camara-Clayette Valérie, Biologist engineer 
• Honoré Aurélie, Technician
• Laporte Mélanie, Technician 
• Lecourt Hélène, Technician
• Louis Maxime, Technician
• Motté Nelly, Technician
• Paume Clélia, Technician
• Richon Catherine, Technician

H2 Molecular biopathology equipment

• 2 2100 Bioanalysers (Agilent®)
• 1 NanoDrop® for sample quantification (Ozyme®)
• 1 Liquidator (Mettler Toledo®)
• 2 QUBIT® sample quantification (Labtech®)
• 6 PCR blocks (96 wells)
• 1 ViiA7 PCR Quantification block, 96 wells (Life Technologies®)
• 2 PCR VERITI® blocks (Life Technologies®)
• 1 DNA Extractor EZ1 (Qiagen®)
• 1 48 Capillary array sequencer shared with Biopath (Life Technologies®)
• 2 PGM IonTorrent® + 3 OneTouch, NG sequencing (Life Technologies®)

Molecular biopathology section

This section performs molecular assays (sequencing of more than 350 targets, PCR, qPCR, NGS etc.) in the context of clinical trials or research studies. This section is also involved in testing for mutations in the context of the Gustave Roussy Personalised Medicine programme.

The skill base of the section has been broadened by developing a team to research knowledge transfer in Haematology.
The nature of blood diseases has led to the development of methods of cell selection designed to isolate sub-populations from tumour samples.

Team members

• Guido Kroemer, Head of the platform
• Sylvère Durand, Analytics manager
• Mélanie Bourgin, Biochimist Post-doct
• Fanny Aprahamian, Technical assistant
• Nitharsshini Nirmalathasan, Apprentice 

Metabolomics platform ressources

The facility consists of four complementary chromatography/mass spectrometry systems as well as Mass Spectrometry Imaging:

• UHPLC U3000 Dionex - Orbitrap q-Exactive Thermo Scientific
• HPLC 1260 Binary Pump Agilent - SelexIon QTRAP 6500+ Sciex
• UHPLC 1290 Binary Pump Agilent - QQQ 6470 Agilent
• GC 7890B - QQQ 7000C Agilent
• DESI-SYNAPT XS Waters

We also have a dedicated sample preparation room, equipped with dryblock concentrator, chemical hoods, speed-vac and -80°C freezer.

Metabolomics platform activities

Mass-spectrometry is the core technology of our facility enabling to measure all potential classes of metabolites for both exploratory and targeted approaches. 

Exploratory metabolomics profiling is performed on a high resolution mass spectrometer (HRMS) coupled to ultra-high performance liquid chromatography (UHPLC).

Targeted metabolomics relies on multiple reaction monitoring (MRM) after separation by UHPLC, gas chromatography (GC), or ion mobility (SelexIon), depending on the properties of the metabolites of interest.

Team members

• Head of the genomic platform: Nathalie DROIN, PhD
  (nathalie.droin@gustaveroussy.fr)
• Marie BRECKLER, Technician
• Betty LEITE, Technician
• Audrey NAIMO, Technician
• Maëla FRANCILLETTE, engineer
• Béatrice FIET, assistant manager

Collective email address: GD-UGF@gustaveroussy.fr

Equipment

• 2 Bioanalyzer 2100 (Agilent)
• 1 FragmentAnalyzer (Agilent)
• 1 S220 Ultrasonicator (Covaris)
• 1 E220 Ultrasonicator (Covaris)

• 1 Robot Bravo (Agilent)
• 1 Robot SpriWorks (Beckman)

• 2 Chromium™ Controller (10X Genomics)
• 1 Tapestri (Mission Bio)

1 NovaSeq6000 (Illumina)

• 1 MiSeq (Illumina)
• 1 MiniSeq (Illumina)
• 1 PromethION 48 (Oxford Nanopore)

Gustave Roussy's US AMMICa Genomics Platform (INSERM US23 / CNRS UMS3655) aims to characterize cancers and experimental models using innovative genomic approaches.